Spectradyne
ARC

Nanoparticle Size, Concentration and Phenotype Analyser

  • Measurement range 50 nm – 10 µm
  • MRPS & simultaneous single-particle fluorescence
  • Counts & sizes individual sizes

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Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11

Spectradynes latest, groundbreaking instrument. Accurate particle size, concentration and phenotype analysis

Fluorescence Microfluidic Resistive Pulse Sensing (F-MRPS, Spectradyne) was used to measure Vesi-Ref CD63 GFP (Vesiculab, UK) without modification and after staining with PE-anti-CD63. F-MRPS detects and measures the size and concentration of nanoparticles using MRPS, an electrical technique, while simultaneously measuring the fluorescence of each particle in up to 3 optical bands. Particle detection and fluorescent measurements are triggered using the MRPS signal, ensuring that each event represents a physical particle larger than the size limit of detection of the cartridge used (65 nm in this case). A one-time factory cross-calibration of the instrument to NIST-traceable MESF standard beads enables particle brightness to be reported by default in traceable units (ERF or MESF when appropriate).

The main figure shows particle concentration vs. size for all particles in the unstained sample (purple), all particles in the stained sample (blue), and subpopulations of the stained sample after gating GFP+ (green), CD63+ (yellow), and double-positive GFP+/CD63+ (red) populations. While the overall particle size distribution displays an approximate power-law dependence of concentration on size that extends to the lower size limit of detection for this cartridge, the fluorescent subpopulations each show the presence of a peak near 75 nm diameter. The 2D scatter plot (inset) shows the brightness of each particle in FITC and PE channels. Absolute concentration and relative abundance of particles are reported for each quadrant, the bounds of which are defined by the fluorescence limits of detection of each channel: 30 FITC ERF and 2.5 PE MESF.

  • Key Features

    • Gives particle size distribution and absolute concentration, as well as single-particle fluorescence
    • Particle sizes: 50 nm to 10 micron diameters
    • Concentration range: approx. 104 — 1012 particles/mL
    • Excitation Laser Wavelength: 488nm
    • Counts & sizes individual particles
    • High resolution size distributions
    • Max particle detection rate: approx. 10,000 particles/s
    • Can measure any material type (transparent & opaque, conducting & insulating)
    • Small bench top footprint
    • Instrument control interface: USB to Windows

  • Technical Specs

    Technology

    Microfluidic resistive pulse sensing and simultaneous single-particle fluorescence. No optical index contrast or other resolution issues
    Cartridges

    Disposable microfluidic cartridges with nanoscale features
    Particle size analysis range

    50 nm to 10 micron diameters
    Concentration range

    approx. 104 — 1012 particles/mL
    Excitation Laser Wavelength

    488nm
    Other

    Bench-top instrument with external laptop or PC
    Instrument Dimensions:

    20″ wide × 20″ deep × 9″ high (500 mm wide × 500 mm deep × 155 mm high)
    Power Supply Dimensions:

    11.8″ wide x 15.6″ deep x 6.1″ high (300mm wide x 396mm deep x 155mm high)
    Weight

    About 30 lbs (14 kg)
    Power:

    110V & 60 Hz | 220V & 50 Hz

  • Applications

    EV’s and Exosomes

    Liposomes and Lipid Nanoparticles

    Virology

    Protein Aggregation

    Industrial Nanoparticles

    Serum

    Gene Therapy

  • Cartridges

    • Eliminates contamination and cleaning requirements
    • Sample volume required: 2-3 microliters
    • Sizing precision: < ±5%
    • On-board filtering: no sample pre-filtering required
    • Compact and easy-to-use cartridge design
    BioLector Microbioreactor

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    We use Meritics for the PM service and installation of all our FlowCams . Megan has been coming to our site for a number of years. She is very efficient in her work, but most of all Megan is very helpful. We have had issues with one of instruments after the install and Megan did her utmost to try and solve it while on site, even though it wasn’t solved Megan has been in contact with the supplier of the instrument to try and solve this problem for us.

    SophieH-2201

  • Job done in a friendly manner

    Booking engineer visits is always easy. David Lowe, Meritics engineer is always very friendly.

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    I have used this company through the laboratory I work for frequently. We specialise in soil analysis, and Meritics were chosen as they have extensive knowledge of the instruments we use. We have several laser particle diffraction analysers with a service contract, and service is always very prompt and meticulous….

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    Meritics have covered the annual servicing of our Multisizer 4e instrument since we purchased it from them just over two years ago. The application scientist who performed the servicing was friendly, knowledgeable, and extremely helpful, happily answering a barrage of questions about how best to use and maintain the instrument. All my interactions with Meritics staff have been positive. I would happily recommend them.

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    After owning one of our instruments for over 8 Years which was purchased from Meritics, it suffered its first breakdown. Upon contacting Meritics, I was instantly put through to an engineer who was very helpful. Upon inspection of the manual, it was decided that it would be more cost effective to have a Meritics engineer visit. The Meritics staff were very communicative and kept me up to date.

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    We had issues with one of the aperture tubes provided by Meritics on a Friday afternoon, while very pressed for a review deadline. Meritics handled our inquiries very fast and professionally and helped us out by shipping us their spare tube to use in the mean time free of charge, while we waited for our new tube to arrive. Communication was very pleasant.

    VeerleB

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    Used Meritics to conduct particle size distribution by laser diffraction. Service as last time, is very quick, well priced and professional.

    I would highly recommend

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