Orflo
Moxi V

Automated Cell Counter

  • True Cell Viability Counts
  • Precision Sizing
  • Highly Accurate
  • Rapid Assays

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Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11

Gold Standard Automated Cell Counter, Cell Size, and Viability

The Orflo Moxi V is your top choice for an Automated Cell Counter due to its exceptional accuracy and efficiency. Utilising Coulter Principle and fluorescence-based technology, it provides precise, reliable results quickly. Its user-friendly interface and high-throughput capability streamline operations, making it ideal for research. With its compact design and robust performance, the Moxi V ensures consistent, high-quality cell analysis, making it a valuable addition to any laboratory.

  • Overview

    The Moxi V Automated Cell Counter provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.

    The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional Cell Analyzers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.

    The Moxi V™ system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No prior Cell Analysis experience is required – you simply just plug and play.

    Some Key Features of the Moxi V include:
    • True Cell Viability Counts – 50-100x more sensitive than vision counters.
    • Precision Sizing – Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
    • Highly Accurate – Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
    • Rapid Assays – Offers a less than 15 second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.

  • Technical Specs

    Id

    MXV102
    Included accessories

    USB power cord, US style USB power adapter, and Type S+ cassette pack
    AC power type

    110 VAC
    Applications

    Gold Standard Cell Count, Cell Size, and Viability
    Battery Type

    Rechargeable 3.7 V, 7500 mAh lithium ion
    Cassette types

    Type S+
    Cell Particle Concentration Range

    10,000 – 5,000 cells/mL Type S+
    Cell types tested

    HEK-293
    HeLa
    PC12
    CD3+T
    CHO-K1
    Cos-7
    HepG2
    Hybridoma
    Jurkat E6-1

    MCF7
    Mesenchymal SC
    Monocyte
    Mouse ESC
    NIH 3T3
    PBMC (cultured)
    Red Blood Cells (RBC)
    L5178y
    C. albicans (Yeast)
    S. cerevisiae Vin 13 (Yeast)
    S. cerevisiae X5 (Yeast)
    Wine Yeast (natural fermentaion)
    S.cerevisiae (Baker’s Yeast
    Safale US-05 Yeast
    Data output formats

    FCS 3.1, screen shots (.bmp), CSV
    Data storage capasity

    4Gb
    Display resolution

    800 x 480 colour touchscreen
    Excitation wavelengths

    532nm
    In British units

    8 lbs
    Intended use statement

    For Research Use Only. Product is not for use in diagnostic procedures
    Laser colour

    Green
    Measurable dynamic range

    3 – 27 microns Type Type S+
    4 – 35 microns Type MF-M
    Measurement time

    10 seconds Type S+
    MPI cell health ratio

    Yes (Size histogram only)
    Number of detection channels flow parameters

    2 colour, 1 size, 1 forward extinction
    Number of PMTs

    1
    Optical detection range

    561nm/LP (e.g. PI)
    Particle size detection method

    Impedimetric (Coulter Principle)
    Pre-programmed tests

    Gold Standard Cell Count, Cell Size, and Viability
    Sample type

    Cell Preparations
    Sample volume

    60 µL
    Supported connectivity

    USB on-the-go
    Useable cell volume

    14 – 10,306 fL Type S+
    14 – 22,449 fL Type MF-M
    Weight

    3.6 kg

  • How it works

    The operating principle behind the Moxi V is a unique combination of Coulter-Principle cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 561nm/LP (e.g. PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is automatically performed by the system but can be easily adjusted/tuned by the user. The resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

    Step 1: Select desired app, insert the cassette and close the doors.


    Step 2: Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.
    Step 3: Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi V, and load Sample 2.
    Data

    Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis package. The actual Moxi V screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi V and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

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  • Outstanding service

    I have been using Meritics for a number of years and they always provide outstanding service and support.
    They have a rapid response turnarounds and are very knowledgeable of the techniques and products they provide.

    LeanneB-375

  • Good service, very helpful

    We use Meritics for the PM service and installation of all our FlowCams . Megan has been coming to our site for a number of years. She is very efficient in her work, but most of all Megan is very helpful. We have had issues with one of instruments after the install and Megan did her utmost to try and solve it while on site, even though it wasn’t solved Megan has been in contact with the supplier of the instrument to try and solve this problem for us.

    SophieH-2201

  • Job done in a friendly manner

    Booking engineer visits is always easy. David Lowe, Meritics engineer is always very friendly.

    ThierryN

  • Service/calibration visit

    Excellent service, arrived on time, polite, friendly and informative.

    NathamM-270

  • 5 Stars

    Excellent service from an excellent company. Would recommend them to anyone.

    BarryH-480

  • Good Service

    David, our service engineer as usual was good at communicating and setting up the recent service appointment, he arrived on time, did the service promptly and informed me of any problems. Friendly and helpful

    TomA-446

  • Superb and prompt service

    I have used this company through the laboratory I work for frequently. We specialise in soil analysis, and Meritics were chosen as they have extensive knowledge of the instruments we use. We have several laser particle diffraction analysers with a service contract, and service is always very prompt and meticulous….

    JamesG-2483

  • Good experience over two years

    Meritics have covered the annual servicing of our Multisizer 4e instrument since we purchased it from them just over two years ago. The application scientist who performed the servicing was friendly, knowledgeable, and extremely helpful, happily answering a barrage of questions about how best to use and maintain the instrument. All my interactions with Meritics staff have been positive. I would happily recommend them.

    RajG-62

  • Fully committed to customer service

    Service second to none, both units serviced and all questions answers by engineer while on site.

    RichardA-786

  • Highly recommend Meritics for equipment purchases and aftercare services

    After owning one of our instruments for over 8 Years which was purchased from Meritics, it suffered its first breakdown. Upon contacting Meritics, I was instantly put through to an engineer who was very helpful. Upon inspection of the manual, it was decided that it would be more cost effective to have a Meritics engineer visit. The Meritics staff were very communicative and kept me up to date.

  • Excellent Service

    Lovely, friendly and extremely helpful. David and his team are always on call with advise and are very easy to work with and are always happy to help.

    NaomiT-64

  • Service of lab equipment

    Excellent service. Friendly and professional service was provided in a timely manner to the expected standard.

    NathanM-270

  • Excellent

    Excellent communication. Updates given well in advance. Very polite staff and engineers.

    NathanM-270

  • Multisizer 4e install at pharmaceutical company

    2 people helped with the install of a Multisizer 4e. They were both very professional and friendly, answering any questions that I had. The installation was quick and the attention to good documentation practice was exceptional.

    Chloel-9

  • Service of Beckman Coulter instruments

    Always a pleasure to welcome the Meritics service engineer, who is knowledgeable, personable and extremely competent.

    NeilT-394

  • Professional

    Friendly and collaborative staff, provided a very good service.

    SamK-403

  • Professional, Customer-oriented, fast service

    We had issues with one of the aperture tubes provided by Meritics on a Friday afternoon, while very pressed for a review deadline. Meritics handled our inquiries very fast and professionally and helped us out by shipping us their spare tube to use in the mean time free of charge, while we waited for our new tube to arrive. Communication was very pleasant.

    VeerleB

  • Very helpful and prompt

    I emailed for some technical help and also to get some spares and both the engineer (David) and purchasing (Sophie) were very prompt to respond and helpful. We do have a service contract but I appreciated being able to email the engineer directly.

    AlexH-474

  • Excellent Service

    Used Meritics to conduct particle size distribution by laser diffraction. Service as last time, is very quick, well priced and professional.

    I would highly recommend

    GavinR-101

  • Excellent and Friendly Service

    Always a pleasure to deal with the people at Meritics Ltd. They understand the importance of our work and the fact that our research projects have deadlines that need to be met. Consequently, problems are dealt with very quickly and professionally and they are willing to go that one step further to make sure their supply chain problems don’t become our problems. Thank you to the team for their support. It is greatly appreciated.

    JanetH-255