VIDEODROP: For a fast characterisation of Extracellular Vesicles following a standard protocol
The recent interest growth in Extracellular Vesicles (EVs) is governed by the potential that these cell derived membranous nanoparticles present in terms of theranostic effect. EV science has now clearly achieved widespread development, as demonstrated by the constantly growing number of EV publications, confirming significant roles of EVs in various physiological pathways like aging, cancer, infectious diseases, and others.
Therefore, there rises an urgent need for both analytical/characterization techniques before proceeding to clinical translation. Up to now, EVs quantification and sizing were achieved by Tunable Resistive Pulse Sensing (TRPS), a complex method relatively time-consuming. Myriade, a French company, developed Videodrop, a new approach for rapid and easy characterization of nanoparticles in a single drop, based on Interferometric Light Microscopy (ILM).
We compared those two methods for EVs characterization on EVs separated from serum and biological liquids: ILM and TRPS. The correlation between the two methods appears to be robust, with high R2 values. These results suggest Videodrop can be a relevant tool for quick characterization of EVs for the study of EVs’ role in physiology and pathology. It is an easy-to-use and fast alternative to the standard more complex and time-consuming methods.
