Characterising tissue culture is crucial for ensuring the reproducibility and quality of biological research. It involves assessing cell morphology, growth rates, genetic stability, and contamination levels. Accurate characterisation ensures that cultured tissues maintain their intended properties, enabling reliable experimental outcomes and facilitating advancements in drug development, regenerative medicine, and biotechnology.
Coulter Counter method
The Coulter Counter method is a widely used technique for analysing tissue culture, providing precise and automated cell counting. It operates on the principle of electrical impedance, where cells suspended in an electrolyte pass through a small aperture, causing measurable changes in electrical resistance. This method allows for accurate determination of cell concentration, size distribution, and viability. It is particularly beneficial for monitoring cell growth and assessing culture health over time. The Coulter Counter method is efficient, reducing manual errors and increasing throughput, making it indispensable for large-scale tissue culture experiments and quality control in biomedical research and industrial applications.
Case study
A research laboratory based in the UK sought to optimise the culture conditions for yeast cells (Saccharomyces cerevisiae) to enhance growth rates and maximise yield for applications in biotechnology and fermentation processes. Precise characterisation of cell growth was crucial for achieving reproducible and high-quality results.
The UK-based research laboratory successfully utilised the Beckman CoulterMultisizer 4e to optimise yeast cell culture conditions and accurately characterise cell growth. This demonstrated significant improvements in biomass yield and culture reproducibility, highlighting the importance of precise cell analysis in biotechnological research and industrial applications. The insights gained from this study pave the way for more efficient and scalable yeast fermentation processes.
