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Particle Size and Count
Beer

Beer, evaluation of final product and filtration efficiency

The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method. A suitable electrolyte solution is required to perform the analysis.The sample is prepared by dissolving a certain volume of beer in the electrolyte and then analysed using a Beckman Coulter Multisizer 3 to determine the size distribution and concentration for the particles present in the beer. The results are reported as number of particles per milliliter for the desired size range.

The use of the Multisizer 3 provides a fast, easy, accurate and automatic method to determine the particle content in beer. The use of this instrument also provides reliable results not dependent on the operator’s judgment making it possible to compare data from different work shifts and/or breweries.

Significance

The determination of particle concentration in beers is important for evaluating and/or correcting several steps during the brewing process and finishing of the product.

■ Evaluation of the Final Product. Each kind of beer has its own characteristics and distinctive flavor; these properties will be influenced to some extent by the content and size distribution of particles present in the final product.The stability and therefore the shelf life of beer are also affected by its particle content.

■ Evaluation of Chill Haze Effect. This is the most common, and in some sense, the most important type of beer hazesince it is relevant to many beer types. As the name suggests, this haze appears when the beer is suitably chilled; the haze disappears upon warming. The temperatures at which the haze appears and disappears depend on the physical stability of the beer.The more stable the beer, the closer to 0 °C before chill haze occurs.The haze involves complexes of highmolecularweight proteins and polyphenols (tannins). These compounds form weak, temperature sensitive hydrogen bonds that are broken as the beer’s temperature increases, allowing the resulting compounds to form a complex with water molecules and go into solution.

■ Filtration Efficiency. Brewers have been using some type of filtration for centuries. If properly used, it can serve as an effective nonadditive tool in beer clarification. Filtration is used in conjunction with fining agents to render beer brilliantly clear and stable with respect to temperature changes. In this paper we will refer to the evaluation of the final product and filtration efficiency.