Moniotoring Plant Cell Cultures with BioLector and Multisizer 4e Instruments
Cell cultures can be used to grow plant cells. Typically, this technique is performed in an aseptic and controlled environment with a variety of nutrient solutions.
Plant cell cultures have a wide range of applications from basic research (e.g., studies on plant growth and differentiation) to mass production of plant-derived metabolites (e.g. production of the anti-cancer agent paclitaxel). Therefore, they have become increasingly attractive and cost-effective alternatives to classical approaches for the mass production of plant-derived metabolites. However, it is crucial to monitor culture conditions to optimise the rate of cell prol iferation and maximize productivity levels. Likewise, the cells must be characterised to detect changes in the cell culture, such as the formation of cell aggregates or cellular lysis. This application note will demonstrate how the BioLector XT microbioreactor and the Multisizer 4e Coulter Counter can be used to optimise plant cell culture conditions and therefore cell growth.
