Monitoring E. coli Cultures with the BioLector and Multisizer 4e Instruments
Escherichia coli (E. coli) is a facultative anaerobic bacterium that lives in the lower intestine of warmblooded
animals, including humans
E. coli can be cultured easily and inexpensively in a laboratory setting
and has become an important model organism in genetics, microbiology and biotechnology. E. coli is
the most common organism used for the large-scale production of therapeutic proteins. Indeed, 30%
of approved therapeutic proteins are currently being produced using E. coli. This application note will
demonstrate how the BioLector microbioreactor and the Multisizer 4e Coulter Counter can be used to
optimize E. coli culture conditions and characterise cell growth