Assessing the particle concentration of mRNA-LNP using Videodrop
Here, we propose to use Videodrop for the analysis of mRNA-LNP and highlight the interest of measuring the particle concentration of mRNA-LNP. The current analyticstrategies employed for mRNA-LNP characterisation lie primarily on size, polydispersity, and zeta-potential measurements. However, particle concentration, evaluated through a single particle measurement technique, is an important parameter to monitor for several reasons:
- • Quality control tests
- • Stability assessment
- • Standardization for comparative studies
When developing new formulations, manufacturing processes, or different storage conditions, quantifying the number of particles enables efficient comparison studies. By comparing particle numbers under different conditions, researchers can assess the impact of the process or composition on transfection activity.
